CD55 antibody (Complement Decay-Accelerating Factor) (FITC)

Details for Product anti-CD55 Antibody No. ABIN94185
Antigen
Reactivity
Human, Non-Human Primate
351
47
15
9
9
3
3
2
2
2
Host
Mouse
256
122
10
7
3
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD55 antibody is conjugated to FITC
50
41
28
12
7
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
257
172
90
87
78
49
37
24
12
12
6
5
3
1
1
1
1
1
1
Options
Immunogen HPB-ALL human T cell line
Clone MEM-118
Isotype IgM
Specificity The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.
HLDA V, WS Code AS S016
Cross-Reactivity (Details) Human, Non-Human Primates
Antigen
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Pathways Complement System, Regulation of Leukocyte Mediated Immunity
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Complement Decay-Accelerating Factor (CD55) antibody (FITC) (ABIN94185) Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 a...
Product cited in: Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).