CD55 antibody (FITC)

Catalog No. ABIN94185

Cited in 1 publication. The FITC-conjugated Mouse Monoclonal anti-CD55 antibody (Clone MEM-118) (ABIN94185) specifically detects CD55 in FACS. The antibody is reactive with Human and Non-Human Primate samples.

Quick Overview for CD55 antibody (FITC) (ABIN94185)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MEM-118

Product Details anti-CD55 Antibody (FITC)

Purpose: Anti-Hu CD55 FITC

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HPB-ALL human T cell line

Isotype: IgM

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD55 antibody (FITC) (ABIN94185)

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, (1999) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: CD55 Molecule (Cromer blood group),CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.,DAF, CROM, CHAPLE

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity