L-Selectin antibody (Selectin L) (FITC)

Details for Product anti-SELL Antibody No. ABIN94211
Antigen
Reactivity
Human
195
165
146
47
15
5
4
3
1
Host
Mouse
219
167
100
9
2
Clonality (Clone)
Monoclonal ()
Conjugate
This L-Selectin antibody is conjugated to FITC
74
69
36
32
9
9
6
5
5
5
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
376
131
117
105
73
51
33
19
14
12
9
7
7
6
5
4
4
3
2
1
1
1
Options
Immunogen Peripheral blood leukocytes
Clone LT-TD180
Isotype IgG1
Specificity The antibody LT-TD180 reacts with CD62L (L-selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes, it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD62L (SELL Antibody Abstract)
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Selectin L (SELL) antibody (FITC) (ABIN94211) Surface staining of human peripheral blood cells with anti-CD62L (LT-TD180) Pacific B...
Product cited in: Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, 2010 (PubMed).

Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009 (PubMed).

Simone, Zicca, Saverino: "The frequency of regulatory CD3+CD8+CD28- CD25+ T lymphocytes in human peripheral blood increases with age." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1454-61, 2008 (PubMed).