CD8 antibody

Catalog No. ABIN94230

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in FACS and IP. Suitable for Human.

Quick Overview for CD8 antibody (ABIN94230)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-87

Product Details anti-CD8 Antibody

Purpose: Anti-Hu CD8 Purified

Specificity: The antibody MEM-87 recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Leukocytes of a patient suffering from LGL Type Leukemia.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2 μg/mL.
Immunoprecipitation: Excellent for immunoisolation of CD8 positive T cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,p32, LEU2