CD9 antibody (CD9) (Biotin)

Details for Product anti-CD9 Antibody No. ABIN94246
Antigen
Reactivity
Human
344
100
52
38
28
27
27
23
19
13
10
9
9
9
9
6
2
1
1
1
Host
Mouse
249
104
40
Clonality (Clone)
Monoclonal ()
Conjugate
This CD9 antibody is conjugated to Biotin
55
49
30
23
5
4
4
4
3
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Microcytotoxicity Assay (MCA), Western Blotting (WB)
297
188
102
57
50
46
38
25
23
22
15
8
6
5
2
2
2
2
2
1
1
1
Options
Immunogen Pre-B cell line NALM-6.
Clone MEM-61
Isotype IgG1
Specificity The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
HLDA VI, WS Code P P-15
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD9 (CD9 Antibody Abstract)
Background CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Pathways Response to Water Deprivation, Cell-Cell Junction Organization
Application Notes Biotinylated antibody is intended for indirect immunofluorescence analysis by flow cytometry and for Western blotting.Suggested working dilution for flow cytometry is 1:400. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD9 (CD9) antibody (Biotin) (ABIN94246) Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9 (M...
Product cited in: Lafleur, Xu, Hemler: "Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 2030-40, 2009 (PubMed).

Singh, Sugimoto, Dhawan, Harris: "Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance." in: American journal of physiology. Cell physiology, Vol. 293, Issue 5, pp. C1660-8, 2007 (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).