CD9 antibody (CD9) (FITC)

Details for Product anti-CD9 Antibody No. ABIN94247
Antigen
Reactivity
Human
344
100
52
38
28
27
27
23
19
13
10
9
9
9
9
6
2
1
1
1
Host
Mouse
249
104
40
Clonality (Clone)
Monoclonal ()
Conjugate
This CD9 antibody is conjugated to FITC
54
49
31
23
5
4
4
4
3
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Microcytotoxicity Assay (MCA), Western Blotting (WB)
297
188
102
57
50
46
38
25
23
22
15
8
6
5
2
2
2
2
2
1
1
1
Options
Immunogen Pre-B cell line NALM-6.
Clone MEM-61
Isotype IgG1
Specificity The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
HLDA VI, WS Code P P-15
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD9 (CD9 Antibody Abstract)
Background CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Pathways Response to Water Deprivation, Cell-Cell Junction Organization
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD9 (CD9) antibody (FITC) (ABIN94247) Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9 (M...
Product cited in: Lafleur, Xu, Hemler: "Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 2030-40, 2009 (PubMed).

Singh, Sugimoto, Dhawan, Harris: "Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance." in: American journal of physiology. Cell physiology, Vol. 293, Issue 5, pp. C1660-8, 2007 (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).