GFAP antibody (Glial Fibrillary Acidic Protein)

Details for Product anti-GFAP Antibody No. ABIN94316
Antigen
Reactivity
Human, Pig (Porcine)
735
400
397
175
161
119
101
27
26
17
15
13
10
8
8
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3
2
2
1
1
1
1
1
Host
Mouse
439
359
28
11
3
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This GFAP antibody is un-conjugated
42
28
26
14
13
13
7
7
7
7
7
7
7
6
5
5
5
5
5
5
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3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
657
417
260
181
175
139
113
62
62
54
50
42
16
16
13
12
7
6
5
5
3
2
1
1
1
1
Options
Immunogen Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Clone GF-02
Isotype IgM
Specificity The antibody GF-02 exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system, it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves.
The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Cross-Reactivity (Details) Human, Porcine
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name GFAP (GFAP Antibody Abstract)
Background Glial Fibrillary Acidic Protein (GFAP) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Glial Fibrillary Acidic Protein (GFAP) antibody (ABIN94316) Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane...
Product cited in: Porchet, Probst, Bouras, Dráberová, Dráber, Riederer: "Analysis of glial acidic fibrillary protein in the human entorhinal cortex during aging and in Alzheimer's disease." in: Proteomics, Vol. 3, Issue 8, pp. 1476-85, 2003 (PubMed).