HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody (Biotin)

Details for Product No. ABIN94381
Antigen
Epitope
C-Term
29
11
8
8
7
7
4
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
43
40
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
184
170
170
110
63
47
35
32
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Clone 5A6G7
Isotype IgG1
Specificity The antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Cross-Reactivity (Details) Human
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes The reagent is intended for Western blotting, ELISA, indirect Flow Cytometry analysis and immunocytochemistry. Suggested working dilution for Western blotting is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody (Biotin) (ABIN94381) Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various...
Product cited in: Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, 2013 (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, 2005 (PubMed).

Le Rond, Gonzalez, Gonzalez, Carosella, Rouas-Freiss: "Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways." in: Immunology, Vol. 116, Issue 3, pp. 297-307, 2005 (PubMed).

Rebmann, Lemaoult, Rouas-Freiss, Carosella, Grosse-Wilde: "Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004." in: Human immunology, Vol. 66, Issue 8, pp. 853-63, 2005 (PubMed).

Le Rond, Le Maoult, Créput, Menier, Deschamps, Le Friec, Amiot, Durrbach, Dausset, Carosella, Rouas-Freiss: "Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients." in: European journal of immunology, Vol. 34, Issue 3, pp. 649-60, 2004 (PubMed).