HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN94384
Antigen
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
185
171
170
110
63
47
35
33
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen Bacterially expressed recombinant HLA-G heavy chain (denatured).
Clone MEM-G-2
Isotype IgG1
Specificity The antibody MEM-G/2 recognizes the free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN94384) Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry stai...
Product cited in: Kren, Slaby, Muckova, Lzicarova, Sova, Vybihal, Svoboda, Fadrus, Lakomy, Vanhara, Krenova, Sterba, Smrcka, Michalek: "Expression of immune-modulatory molecules HLA-G and HLA-E by tumor cells in glioblastomas: An unexpected prognostic significance?" in: Neuropathology : official journal of the Japanese Society of Neuropathology, 2010 (PubMed).

Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." in: Human immunology, Vol. 64, Issue 11, pp. 1039-44, 2003 (PubMed).

Poláková, Bandzuchová, Hofmeister, Weiss, Hutter, Russ: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).