kappa Light Chain antibody

Catalog No. ABIN94406

The Mouse Monoclonal anti-kappa Light Chain antibody has been validated for FACS, WB, IHC (p), ELISA, IP and ICC. It is suitable to detect kappa Light Chain in samples from Human.

Quick Overview for kappa Light Chain antibody (ABIN94406)

Target: kappa Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This kappa Light Chain antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: MEM-09

Product Details anti-kappa Light Chain Antibody

Purpose: Anti-Hu Ig Kappa Light Chain Purified

Specificity: The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Crude thymus membrane fraction.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for kappa Light Chain

Target: kappa Light Chain

Alternative Name: Ig Kappa Light Chain

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk