TCR Vbeta5.3-Related antibody

Catalog No. ABIN94477

This Mouse Monoclonal antibody specifically detects in FACS, IP and WB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for TCR Vbeta5.3-Related antibody (ABIN94477)

Target: TCR Vbeta5.3-Related

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Clone: MEM-262

Product Details

Purpose: Anti-Hu TCR Vbeta5.3-related Purified

Specificity: The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Cross-Reactivity (Details): Other not tested, Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human thymoma cell line HPB-ALL.

Isotype: IgG2a

Application Details

Application Notes: Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Pavlistová, Drbal, Hilgert, Horejsí: "A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).

Target Details

Target: TCR Vbeta5.3-Related

Background: TCRVB5.3-related