B-Cell Linker (BLNK) (AA 171-356) antibody

Details for Product No. ABIN94495
Antigen
Epitope
AA 171-356
40
36
15
15
15
12
10
10
8
6
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
206
117
79
7
3
2
2
2
1
1
1
Host
Rabbit
187
18
7
Clonality
Polyclonal
Conjugate
Un-conjugated
11
8
8
7
7
7
7
7
7
4
4
4
4
4
4
4
3
1
Application
Western Blotting (WB)
137
77
52
51
32
17
11
7
3
2
2
1
1
Options
Immunogen A fusion protein representing amino acids 171-356 of human BLNK.
Specificity The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
Cross-Reactivity (Details) Mouse
Purification Purified from rabbit serum by precipitation methods
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name SLP65 / BLNK (BLNK Antibody Abstract)
Background SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa, B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptor proteins, thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-B-Cell Linker (BLNK) (AA 171-356) antibody (ABIN94495) Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse m...