Kinesin (AA 331-906) antibody

Details for Product No. ABIN94498
Antigen
Epitope
AA 331-906
15
4
3
2
1
Reactivity
Human, Pig (Porcine), Mouse (Murine)
25
22
7
6
4
Host
Rabbit
21
11
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
15
13
8
6
5
4
4
4
3
3
Options
Immunogen stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)
Specificity The polyclonal antibody detects total level of endogenous kinesin protein.
Cross-Reactivity (Details) Human, Mouse, Porcine, Other not tested
Purification Purified from rabbit serum by affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Background Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Kinesin (AA 331-906) antibody (ABIN94498) Detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain ...
Product cited in: Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, 2005 (PubMed).