Acetylcholinesterase antibody (Biotin)

Catalog No. ABIN94540

Supplier Product No.: 200-106-232-0100

This anti-Acetylcholinesterase antibody is a Goat Polyclonal antibody detecting Acetylcholinesterase in WB, ELISA and IP. Suitable for Cow.

Quick Overview for Acetylcholinesterase antibody (Biotin) (ABIN94540)

Target: Acetylcholinesterase (AChE)

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Acetylcholinesterase Antibody (Biotin)

Supplier Product No.: 200-106-232-0100

Supplier: Rockland

Purpose: Acetyl Cholinesterase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].

Characteristics: Synonyms: goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE

Purification: Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: ACHE

Background: Background: Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research.

Gene ID: 540446

NCBI Accession: NP_001069688

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development