ADA antibody
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- Target See all ADA Antibodies
- ADA (Adenosine Deaminase (ADA))
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Reactivity
- Human, Mouse, Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ADA antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 100-401-140
- Supplier
- Rockland
- Purpose
- Adenosine Deaminase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Adenosine Deaminase [Calf Spleen].
- Characteristics
- Synonyms: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Adenosine Deaminase [Calf Spleen]
Immunogen Type: Native Protein
- Top Product
- Discover our top product ADA Primary Antibody
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- Application Notes
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Application Note: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:10,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 90 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- ADA (Adenosine Deaminase (ADA))
- Alternative Name
- ADA (ADA Products)
- Background
- Background: Adenosine Deaminase catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. It plays an important role in purine metabolism and in adenosine homeostasis. Adenosine Deaminase modulates signaling by extracellular adenosine, therefore contributes indirectly to cellular signaling events. It acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Adenosine Deaminase enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. It enhances CD4+ T-cell differentiation and proliferation. And acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. It stimulates plasminogen activation. It also plays a role in male fertility and a protective role in early post implantation embryonic development.
- Gene ID
- 280712
- NCBI Accession
- NP_776312
- UniProt
- P56658
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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