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ADA antibody

ADA Reactivity: Human, Mouse, Cow WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN94705
Supplier Product No.: 100-401-140
  • Target See all ADA Antibodies
    ADA (Adenosine Deaminase (ADA))
    Reactivity
    • 60
    • 33
    • 17
    • 13
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Cow
    Host
    • 71
    • 19
    • 1
    Rabbit
    Clonality
    • 74
    • 17
    Polyclonal
    Conjugate
    • 47
    • 8
    • 6
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADA antibody is un-conjugated
    Application
    • 80
    • 40
    • 13
    • 13
    • 12
    • 11
    • 11
    • 10
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    100-401-140
    Supplier
    Rockland
    Purpose
    Adenosine Deaminase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Adenosine Deaminase [Calf Spleen].
    Characteristics
    Synonyms: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Adenosine Deaminase [Calf Spleen]

    Immunogen Type: Native Protein

    Top Product
    Discover our top product ADA Primary Antibody
  • Application Notes

    Application Note: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    90 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    ADA (Adenosine Deaminase (ADA))
    Alternative Name
    ADA (ADA Products)
    Background
    Background: Adenosine Deaminase catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. It plays an important role in purine metabolism and in adenosine homeostasis. Adenosine Deaminase modulates signaling by extracellular adenosine, therefore contributes indirectly to cellular signaling events. It acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Adenosine Deaminase enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. It enhances CD4+ T-cell differentiation and proliferation. And acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. It stimulates plasminogen activation. It also plays a role in male fertility and a protective role in early post implantation embryonic development.
    Gene ID
    280712
    NCBI Accession
    NP_776312
    UniProt
    P56658
    Pathways
    Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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