ADA antibody

Catalog No. ABIN94705

Supplier Product No.: 100-401-140

Product Details anti-ADA Antibody

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 100-401-140

Supplier: Rockland

Purpose: Adenosine Deaminase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Adenosine Deaminase [Calf Spleen].

Characteristics: Synonyms: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Adenosine Deaminase [Calf Spleen]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA (ADA Products)

Background: Background: Adenosine Deaminase catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. It plays an important role in purine metabolism and in adenosine homeostasis. Adenosine Deaminase modulates signaling by extracellular adenosine, therefore contributes indirectly to cellular signaling events. It acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Adenosine Deaminase enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. It enhances CD4+ T-cell differentiation and proliferation. And acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. It stimulates plasminogen activation. It also plays a role in male fertility and a protective role in early post implantation embryonic development.

Gene ID: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process