ALT antibody
-
- Target See all ALT Antibodies
- ALT (Alanine Aminotransferase (ALT))
-
Reactivity
- Pig
-
Host
-
Sheep
-
Clonality
- Polyclonal
-
Conjugate
- This ALT antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 100-601-206
- Supplier
- Rockland
- Purpose
- Alanine Transaminase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alanine Transaminase (ALT) [Pig Heart].
- Characteristics
- Synonyms: sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
-
Immunogen: Alanine Transaminase (ALT) [Pig Heart]
Immunogen Type: Native Protein
- Top Product
- Discover our top product ALT Primary Antibody
-
-
- Application Notes
-
Application Note: Anti-Alanine Transaminase has been assayed against 1.0 μg of Alanine Transaminase (ALT) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 96 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- ALT (Alanine Aminotransferase (ALT))
- Alternative Name
- GPT (ALT Products)
- Background
- Background: Alanine Transaminase catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
- Gene ID
- 2875, 113565
- UniProt
- P13191
-