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ALT antibody

ALT Reactivity: Pig WB, ELISA Host: Sheep Polyclonal unconjugated
Rockland
Catalog No. ABIN94798
Supplier Product No.: 100-601-206
  • Target See all ALT Antibodies
    ALT (Alanine Aminotransferase (ALT))
    Reactivity
    • 51
    • 24
    • 19
    • 13
    • 7
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Pig
    Host
    • 66
    • 12
    • 4
    • 1
    Sheep
    Clonality
    • 70
    • 12
    Polyclonal
    Conjugate
    • 48
    • 17
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ALT antibody is un-conjugated
    Application
    • 70
    • 36
    • 27
    • 13
    • 11
    • 8
    • 8
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA
    Supplier Product No.
    100-601-206
    Supplier
    Rockland
    Purpose
    Alanine Transaminase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alanine Transaminase (ALT) [Pig Heart].
    Characteristics
    Synonyms: sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Alanine Transaminase (ALT) [Pig Heart]

    Immunogen Type: Native Protein

    Top Product
    Discover our top product ALT Primary Antibody
  • Application Notes

    Application Note: Anti-Alanine Transaminase has been assayed against 1.0 μg of Alanine Transaminase (ALT) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    96 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    ALT (Alanine Aminotransferase (ALT))
    Alternative Name
    GPT (ALT Products)
    Background
    Background: Alanine Transaminase catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
    Gene ID
    2875, 113565
    UniProt
    P13191
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