ADH1 antibody

Catalog No. ABIN94889

Supplier Product No.: 100-4143

The Rabbit Polyclonal anti-ADH1 antibody has been validated for WB, IP and ELISA. It is suitable to detect ADH1 in samples from Saccharomyces cerevisiae.

Quick Overview for ADH1 antibody (ABIN94889)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-ADH1 Antibody

Supplier Product No.: 100-4143

Supplier: Rockland

Purpose: Alcohol Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alcohol Dehydrogenase [Yeast].

Characteristics: Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Alcohol Dehydrogenase Antibody is suitable for use in ELISA and western blot.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: ADH1

Background: Background: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone.  Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer.  This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization.  Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.

Gene ID: 2538902, 12643994

UniProt: P00330