Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN94938

Supplier Product No.: 100-6135

This anti-Intestinal Alkaline Phosphatase antibody is a Sheep Polyclonal antibody detecting Intestinal Alkaline Phosphatase in WB and ELISA. Suitable for Cow.

Quick Overview for Intestinal Alkaline Phosphatase antibody (ABIN94938)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Supplier Product No.: 100-6135

Supplier: Rockland

Purpose: Alkaline Phosphatase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alkaline Phosphatase [Calf intestine].

Characteristics: Synonyms: sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Alkaline Phosphatase [Calf intestine]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Alkaline Phosphatase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 33.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: Calf Intenstinal Alkaline Phosphatase

Background: Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.

Gene ID: 280993

UniProt: P19111