ATP6V1B1 antibody (Middle Region)

Catalog No. ABIN950569

This Rabbit Polyclonal antibody specifically detects ATP6V1B1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for ATP6V1B1 antibody (Middle Region) (ABIN950569)

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATP6V1B1 Antibody

Binding Specificity: AA 291-318, Middle Region

Specificity: This antibody reacts to ATP6V1B1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 291-318 amino acids from the Central region of human ATP6V1B1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP6V1B1

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Alternative Name: ATP6V1B1

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].Synonyms: ATP6B1, Endomembrane proton pump 58 kDa subunit, V-ATPase subunit B 1, V-type proton ATPase subunit B, VATB, VPP3, Vacuolar proton pump subunit B 1, kidney isoform

Gene ID: 525

NCBI Accession: NP_001683

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport