GLB1 antibody

Catalog No. ABIN950653

Product Details anti-GLB1 Antibody

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: E. coli

Host: Chicken

Clonality: Polyclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes the LacZ gene product with high titre and high specificity. This anitbody can be used together with Rabbit and Mouse primaty antibodies for Double Immunostaining with no Secondary antibody cross reactivity. It even works well with fusion protein of LacZ! Quality Control: This antibody preparation was analyzed by Immunohistochemistry (at a dilution of 1/5000) using tissues infected with a virus bearing the LacZ gene. Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FC-N, 1/2000 dilution) was used as a secondary antibody.

Purification: Purified

Immunogen: Purified Beta-Galactosidase (LacZ) protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using Beta-Galactosidase conjugated to an Agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 myg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.0 mg/mL (based on absorbance at 280 nm)

Buffer: Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C in the dark.

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: beta Galactosidase (GAL) (GLB1 Products)

Background: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

Gene ID: 945006

NCBI Accession: AP_000996

Pathways: Glycosaminoglycan Metabolic Process