CD25 antibody (Biotin)

Catalog No. ABIN951247

This anti-CD25 antibody is a Rat Monoclonal antibody detecting CD25 in FACS, IP and IHC (fro). Suitable for Mouse.

Quick Overview for CD25 antibody (Biotin) (ABIN951247)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PC61-5-3

Product Details anti-CD25 Antibody (Biotin)

Specificity: Anti-mouse CD25 (IL-2R) monoclonal antibody reacts with the low affinity alpha chain of the interleukin-2 receptor antigen present on activated T and B cells in mice. CL8925B inhibits IL-2 binding and IL-2 dependent proliferation.

Purification: Affinity chromatography on Protein G

Immunogen: B6.1 CTL Cell line Donor: OFA rat spleen Fusion Partner: Mouse myeloma line P3X63Ag8.653

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation