Transferrin Receptor antibody (FITC)

Catalog No. ABIN951377

Product Details anti-Transferrin Receptor Antibody (FITC)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the Mouse Transferrin Receptor protein on the surface of most dividing cells. Suitable for subset analysis of the developmental stage of mouse cells and as a proliferation marker.

Characteristics: Synonyms: TfR1, p90, Transferrin receptor protein 1

Purification: Protein G Chromatography

Clone: R17217-1-4

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry (1 g to stain 1.0 x 10^6 cells). Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.4 containing 1 % BSA and 0.09 % Sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE! Centrifuge vial before opening. This productis photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC (TFRC Products)

Background: Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 22042

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis