C5AR1 antibody (Biotin)

Catalog No. ABIN951419

The Rat Monoclonal anti-C5AR1 antibody is suitable to detect C5AR1 in samples from Human. It has been validated for FACS.

Quick Overview for C5AR1 antibody (Biotin) (ABIN951419)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This C5AR1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 8D6

Product Details anti-C5AR1 Antibody (Biotin)

Specificity: This monoclonal antibody detects an epitope on the N terminus (aa 1-15) of the human C5a receptor (also known as CD88) found on human myeloid cells including granulocytes and monocytes/macrophages. It does not bind to tubular epithelial cells in situ under normal or inflammatory conditions. C5aR binds to C5a, a 74 aa peptide cleaved from complement protein C5. C5a receptor has seven transmembrane segments and is a member of the rhodopsin subfamily of G protein coupled receptors.

Purification: Affinity chromatography on Protein G

Immunogen: RBL-2H3 (rat basophilic leukemia) transfected cells expressing human C5aR on the cell surface Donor: Lou/c rat spleen cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: CD88 / C5R1

Background: Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

Gene ID: 728

NCBI Accession: NP_001727

UniProt: P21730

Pathways: Complement System