MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87)

Details for Product anti-MAP3K5 Antibody No. ABIN95283
Antigen
Epitope
AA 76-87, pSer83
68
56
28
20
20
16
15
15
15
10
9
9
7
7
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
423
192
108
8
5
1
Host
Rabbit
404
21
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
16
11
11
11
11
11
11
11
10
5
5
5
5
5
5
4
4
2
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
302
149
132
74
65
41
20
18
17
5
5
3
2
2
1
Options
Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name ASK-1 (MAP3K5 Antibody Abstract)
Background ASK-1 (apoptosis signal-regulating kinase 1 - also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors.   Overexpression of ASK-1 induces apoptotic cell death.  ASK-1 is expressed in a variety of human and mouse tissues.  The overall amino acid sequence identity between the mouse and human ASK1 is 91.9%.  ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1).  Please refer to the reference list at the end of this document for further information.
Synonyms: Apoptosis signal regulating kinase 1 antibody, ASK 1 antibody, ASK1 antibody, MAP/ERK kinase kinase 5 antibody, MAP3K5 antibody, MAPK/ERK kinase kinase 5 antibody
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response
Application Notes This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended.  A 155 kDa band corresponding to human ASK-1 is detected.  Whole cell lysates from SW1353 can be used as a positive control.  For ELISA a 1:5,000 to 1:10,000 dilution is recommended.   Researchers should determine optimal titers for other applications.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 76-87), (pSer83) antibody (ABIN95283) Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ...
Image no. 2 for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 76-87), (pSer83) antibody (ABIN95283) ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested again...
Product cited in: Subramanyam, Takahashi, Hasegawa, Mohri, Okada: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, 2010 (PubMed).