KDM3B antibody (Middle Region)

Catalog No. ABIN952970

The Rabbit Polyclonal anti-KDM3B antibody has been validated for WB, FACS and EIA. It is suitable to detect KDM3B in samples from Human.

Quick Overview for KDM3B antibody (Middle Region) (ABIN952970)

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-KDM3B Antibody

Binding Specificity: AA 876-905, Middle Region

Specificity: This antibody recognizes Human JMJD1B / KDM3B (Center).

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 876-905 amino acids from the Central region of human JHDM2b Genename: KDM3B

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KDM3B

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Alternative Name: JMJD1B / KDM3B

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.Synonyms: 5qNCA, C5orf7, JHDM2B, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, KIAA1082, Lysine-specific demethylase 3B, Nuclear protein 5qNCA

Molecular Weight: 84kd

Gene ID: 51780

NCBI Accession: NP_057688

Pathways: Warburg Effect