MAP4K1 antibody

Catalog No. ABIN953299

This Mouse Monoclonal antibody specifically detects MAP4K1 in WB, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for MAP4K1 antibody (ABIN953299)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 2A1

Product Details anti-MAP4K1 Antibody

Specificity: Recognizes MAP4K1

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulfate Precipitation

Immunogen: Recombinant Human protein purified from E. coli (His/ABD-HPK1).

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % Sdium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1

Background: HPK1, a KHS protein kinase, is an upstream activator of the stress-activated protein kinase (SAPK) signaling pathway. The protein has a structure similar to other GCK (Group I) kinases and contains four consensus SH3 binding motifs, which have been shown to interact with the SH3 motifs of the MAP kinase kinase kinases MLK3 and MEKK1 and several adapter proteins that couple to tyrosine kinases, including BASH, Blnk, Clnk, Crk, CrkL and Grb2. Additionally, HPK1 has been shown to be phosphorylated and activated by the tyrosine kinase c-Abl in response to genotoxic agents. At least two mRNA transcripts have been reported.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1

Gene ID: 11184

NCBI Accession: NP_001036065

UniProt: Q92918

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor