MED14 antibody (Middle Region)

Catalog No. ABIN953367

This anti-MED14 antibody is a Rabbit Polyclonal antibody detecting MED14 in WB and EIA. Suitable for Human and Mouse.

Quick Overview for MED14 antibody (Middle Region) (ABIN953367)

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED14 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MED14 Antibody

Binding Specificity: AA 585-615, Middle Region

Specificity: This antibody recognizes Human and Mouse MED14 (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 585-615 amino acids from the Central region of human MED14

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MED14

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Alternative Name: MED14

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.Synonyms: ARC150, Activator-recruited cofactor 150 kDa component, CRSP complex subunit 2, CRSP2, CXorf4, Cofactor required for Sp1 transcriptional activation subunit 2, DRIP150, EXLM1, Mediator of RNA polymerase II transcription subunit 14, RGR1, TRAP170 Mediator complex 14, Thyroid hormone receptor-associated protein complex 170 kDa component, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component

Molecular Weight: 160607 Da

Gene ID: 9282

NCBI Accession: NP_004220

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha