Myeloid Lineage antibody (Biotin)

Catalog No. ABIN953565

Product Details

Target: Myeloid Lineage

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Protein G Chromatography of Ascites fluid.

Immunogen: Anemic Rat bone marrow.

Clone: OX-82

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer.

Target Details

Target: Myeloid Lineage

Background: Hematopoietic stem cells (HSC) are the precursor cells found in the bone marrow which give rise to all the blood cell types of both the Myeloid and lymphoid lineages, which include monocytes and macrophages, neutrophils, basophils, eosinophils, T cells, B cells, NK cells, microglia, erythrocytes, megakaryocytes and dendritic cells. During the process of hematopoiesis, Myeloid lineage cells originate from the bone marrow, while Lymphoid lineage cells originate from the lymph tissue. Blimp-1 is a key regulator of the differentiation of the separate hematopoietic myeloid and lymphoid lineages. The distinction between myeloid and lymphoid lineages is essential to diagnose and treat certain cancers. Myeloid lineage cells induce inflammatory cytokine production upon activation by Kaposi's sarcoma-associated herpesvirus OX2 glycoprotein. At the stage of myelocytes, Myeloid lineage cells express a substantial number of IL-8 receptor homologs.