Netrin 1 antibody

Catalog No. ABIN953654

Product Details anti-Netrin 1 Antibody

Target: Netrin 1 (NTN1)

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Recognizes Netrin-1.

Purification: Affinity Chromatography using a peptide column.

Immunogen: Synthetic peptide KLH conjugated corresponding to the netrin-1 gene product, but was shared between the Human (O95631) and Mouse (O9118) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: Netrin-1 (NTN1 Products)

Synonyms: ntn1-A antibody, Netrin-1 antibody, NTN1 antibody, unc-6 antibody, MGC147073 antibody, NTN1L antibody, AI561871 antibody, BC019633 antibody, netrin-1 antibody, netrin 1 L homeolog antibody, netrin 1 antibody, ntn1.L antibody, NTN1 antibody, ntn1 antibody, Ntn1 antibody

Background: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Netrin 1's main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity. Murine Netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various nonneural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of "commissural neurons" from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.Synonyms: NTN1, NTN1L

Gene ID: 9423

NCBI Accession: NP_004813

Pathways: Regulation of Cell Size