NeuN antibody

Catalog No. ABIN953655

This anti-NeuN antibody is a Chicken Polyclonal antibody detecting NeuN in IF and IHC (p). Suitable for Human and Mouse.

Quick Overview for NeuN antibody (ABIN953655)

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NeuN Antibody

Specificity: Recognizes Neu-N antigen (also known as Fox-3).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Synthetic peptide KLH conjugated corresponding to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the Human (NP_001076044) and Mouse (NP_001020102) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 50 % Glycerol and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: RBFOX3

Background: Human Neu-N (Fox3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons, and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and Mouse antibodies against various cytoplasmic neuronal antigens.Synonyms: FOX3, Fox-1 homolog C, NeuN, RNA binding protein fox-1 homolog 3

Gene ID: 146713

NCBI Accession: NP_001076044