Aspartate Transaminase (Ast) antibody

Details for Product No. ABIN95405
Antigen
Reactivity
Pig (Porcine)
4
Host
Sheep
4
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
Application
ELISA
4
4
Options
Immunogen Aspartate Aminotransferase (AST) [Pig Heart]
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Antigen
Alternative Name Aspartate Transaminase
Background Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
Gene ID 396967
UniProt P00503
Application Notes This product has been assayed against 1.0 ug of Aspartate Aminotransferase (AST) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. 
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 96 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Mikami, Shibuya, Kimura, Nagahara, Yamada, Kimura: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (1:3000).