PIGA antibody (C-Term)

Catalog No. ABIN954134

The Rabbit Polyclonal anti-PIGA antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PIGA in samples from Human.

Quick Overview for PIGA antibody (C-Term) (ABIN954134)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIGA Antibody

Binding Specificity: AA 147-177, C-Term

Specificity: This antibody recognizes PIGA at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 147-177aa) of human PIGA.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: PIGA

Background: The PIGA gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.Synonyms: GlcNAc-PI synthesis protein, PIG-A, Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, Phosphatidylinositol-glycan biosynthesis class A protein

Gene ID: 5277

NCBI Accession: NP_002632

Pathways: Inositol Metabolic Process