PIGA antibody (AA 179-228)

Catalog No. ABIN6744797

Product Details anti-PIGA Antibody

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Binding Specificity: AA 179-228

Reactivity: Human, Mouse, Cow, Pig, Rabbit, Dog, Horse, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PIGA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Rat, Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human PIGA (P37287, NP_002632). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus, Lizard (100%), Rat, Hamster, Turkey, Zebra finch, Chicken, Zebrafish (92%), Xenopus (90%), Guinea pig, Stickleback (85%), Panda (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: GPI3 / PIGA (PIGA Products)

Synonyms: GPI3 antibody, MCAHS2 antibody, PIG-A antibody, AI194334 antibody, Pig-a antibody, im:6911675 antibody, zgc:56589 antibody, phosphatidylinositol glycan anchor biosynthesis class A antibody, phosphatidylinositol glycan anchor biosynthesis, class A antibody, PIGA antibody, Piga antibody, piga antibody

Background: Name/Gene ID: PIGA

Synonyms: PIGA, GPI anchor biosynthesis, GPI3, PIG-A, GlcNAc-PI synthesis protein, MCAHS2

Gene ID: 5277

NCBI Accession: NP_002632

UniProt: P37287

Pathways: Inositol Metabolic Process