SPI1 antibody (C-Term)

Catalog No. ABIN954927

This anti-SPI1 antibody is a Rabbit Polyclonal antibody detecting SPI1 in WB and EIA. Suitable for Human.

Quick Overview for SPI1 antibody (C-Term) (ABIN954927)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SPI1 Antibody

Binding Specificity: AA 216-246, C-Term

Specificity: This antibody recognizes SPI1 / Transcription factor PU.1.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 216-246aa) of human SPI1 / Transcription factor PU.1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1 / PU.1

Background: The SPI1 gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: 31 kDa transforming protein, SFPI1, SPI-1 proto-oncogene, Transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1

Gene ID: 6688

NCBI Accession: NP_001074016

Pathways: Stem Cell Maintenance