TUBB3 antibody

Catalog No. ABIN955393

This Chicken Polyclonal antibody specifically detects TUBB3 in WB, IF and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for TUBB3 antibody (ABIN955393)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TUBB3 Antibody

Specificity: Recognizes Tubulin beta-3.

Purification: Affinity Chromatography.

Immunogen: Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3 / TUBB4

Background: Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III

Gene ID: 10381

NCBI Accession: NP_001184110

Pathways: Microtubule Dynamics, M Phase