Uromodulin antibody (HRP)

Catalog No. ABIN955476

Product Details anti-Uromodulin Antibody (HRP)

Target: Uromodulin (UMOD)

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Uromodulin antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Specificity: Anti-Human Tamm-Horsfall protein (THP) is a monoclonal antibody which reacts with an epitope of the urinary mucoprotein. This monoclonal antibody has shown good localization to the distal tubule and collecting duct as well as to large deposits of Tamm-Horsfall protein which has clogged peri-pelvic veins. This antibody has been tested against 21 different Human proteins in Solid Phase Radioimmunoassay and has been shown to be specific for Tamm-Horsfall protein only. Results of ELISA show no visible reaction with Human albumin or transferrin. Also no staining of Human tissue other than that noted for kidney sections has been observed.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Human Tamm-Horsfall protein.

Clone: 01-10-32

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 50 % Glycerol (v/v)., 4-5 mg/mL BSA

Precaution of Use: Avoid exposure of conjugate to Sodium Azide or Thimerosal, as these can inhibit peroxidase activity.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted at (in aliquots) at -20 °C. This product is photosensitive and should be protected from light.

Target Details for Uromodulin

Target: Uromodulin (UMOD)

Abstract: UMOD Products

Synonyms: ADMCKD2 antibody, FJHN antibody, HNFJ antibody, HNFJ1 antibody, MCKD2 antibody, THGP antibody, THP antibody, urehr4 antibody, uromodulin antibody, UMOD antibody, Umod antibody

Background: Tamm-Horsfall Protein (THP) is a glycoprotein of approximately 80 kDa containing up to 25 % carbohydrate by weight. It exists as a high molecular weight polymer in urine and approximately 50 mg of the THP are excreted per day by adult humans. THP is produced by the epithelial cells lining the thick ascending loop of Henle and distal convoluted tubules of the kidney. THP is also the primary constituent of renal tubular casts and THP aggregation has been implicated as a factor in the pathogenesis of acute renal failure by causing tubular obstruction. Antibody to THP is present in the serum of patients with pyelonephritis and reflus uropathy.Synonyms: THP, Tamm-Horsfall urinary glycoprotein

Gene ID: 7369

NCBI Accession: NP_001008390

UniProt: P07911