XBP1 antibody (C-Term)

Catalog No. ABIN955595

The Rabbit Polyclonal anti-XBP1 antibody is suitable to detect XBP1 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for XBP1 antibody (C-Term) (ABIN955595)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-XBP1 Antibody

Binding Specificity: AA 224-252, C-Term

Specificity: This antibody detects XBP1 / TREB5 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Protein A column followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 224-252 amino acids from the C-terminal region of human XBP1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 / TREB5

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gene ID: 7494

NCBI Accession: NP_001073007

Pathways: ER-Nucleus Signaling, Unfolded Protein Response