ZP4 antibody (C-Term)

Catalog No. ABIN955755

The Rabbit Polyclonal anti-ZP4 antibody (ABIN955755) specifically detects ZP4 in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for ZP4 antibody (C-Term) (ABIN955755)

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ZP4 Antibody

Binding Specificity: AA 452-482, C-Term

Specificity: This antibody detects ZP4 / ZPB (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A column followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human ZP4

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.

Target Details for ZP4

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Alternative Name: ZP4 / ZPB

Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.Synonyms: Zona pellucida protein B, Zona pellucida sperm-binding protein 4

Gene ID: 57829

NCBI Accession: NP_067009