Biotin antibody

Catalog No. ABIN95606

Supplier Product No.: 100-1198

Product Details anti-Biotin Antibody

Target: Biotin

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-1198

Supplier: Rockland

Purpose: Biotin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotinylated IgG and Biotinylated Bovine Serum Albumin (BSA).

Characteristics: Synonyms: Goat anti-Biotin Antibody, Goat anti Biotin

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Western Blot Dilution: 1:10,000 - 1:50,000

ELISA Dilution: 1:100,000 - 1:500,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.