Biotin antibody (Alkaline Phosphatase (AP))
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- Target See all Biotin products
- Biotin
- Reactivity
- Please inquire
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Biotin antibody is conjugated to Alkaline Phosphatase (AP)
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-105-098
- Supplier
- Rockland
- Purpose
- Biotin Antibody Alkaline Phosphatase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
- Characteristics
- Synonyms: goat anti-Biotin Antibody Alkaline Phosphatase Conjugation, Alk Phos conjugated goat anti-Biotin Antibody
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Biotin Alkaline Phosphatase conjugated antibody has been tested by ELISA an is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:3,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Alkaline Phosphatase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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