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Biotin antibody (Alkaline Phosphatase (AP))

WB, ELISA, IHC Host: Goat Polyclonal Alkaline Phosphatase (AP)
Rockland
Catalog No. ABIN95612
Supplier Product No.: 600-105-098
  • Target See all Biotin products
    Biotin
    Reactivity
    Please inquire
    Host
    • 62
    • 38
    • 31
    Goat
    Clonality
    • 83
    • 40
    • 1
    Polyclonal
    Conjugate
    • 36
    • 14
    • 8
    • 8
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Biotin antibody is conjugated to Alkaline Phosphatase (AP)
    Application
    • 78
    • 56
    • 51
    • 46
    • 39
    • 33
    • 16
    • 13
    • 12
    • 9
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-105-098
    Supplier
    Rockland
    Purpose
    Biotin Antibody Alkaline Phosphatase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
    Characteristics
    Synonyms: goat anti-Biotin Antibody Alkaline Phosphatase Conjugation, Alk Phos conjugated goat anti-Biotin Antibody
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-Biotin Alkaline Phosphatase conjugated antibody has been tested by ELISA an is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

    Western Blot Dilution: 1:500 - 1:2,500

    ELISA Dilution: 1:3,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Expiry Date
    12 months
  • Target
    Biotin
    Abstract
    Biotin Products
    Target Type
    Chemical
    Background
    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Alkaline Phosphatase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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