UNG antibody (AA 281-298)

Catalog No. ABIN959421

This anti-UNG antibody is a Rabbit Polyclonal antibody detecting UNG in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for UNG antibody (AA 281-298) (ABIN959421)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UNG Antibody

Binding Specificity: AA 281-298

Brand: IHC-plus™

Specificity: The synthetic peptide sequence used for immunogen is 100 % conserved between UNG1 and UNG2. Thus recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kD, 27.5 kD, 31 kD, and 38 kD forms have been described (Bharati et al., 1998, Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Marmoset, Mouse, Dog, Bat, Bovine, Horse, Rabbit, Turkey, Chicken (94%) Rat, Hamster, Elephant, Panda, Pig, Platypus (89%) Zebrafish (83%).

Purification: Protein G purified

Immunogen: Synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Marmoset, Mouse, Dog, Bat, Bovine, Horse, Rabbit, Turkey, Chicken (94%), Rat, Hamster, Elephant, Panda, Pig, Platypus (89%), Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Alternative Name: UNG / Uracil DNA Glycosylase

Background: Name/Gene ID: UNG

Synonyms: UNG, DGU, HIGM4, HIGM5, Uracil-DNA glycosylase, UNG1, UNG2, UDG, UNG15

Gene ID: 7374

UniProt: P13051

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response