Smooth Muscle Actin antibody

Catalog No. ABIN959463

This anti-Smooth Muscle Actin antibody is a Mouse Monoclonal antibody detecting Smooth Muscle Actin in WB, IHC, ELISA, IHC (fro) and IEM. Suitable for Human, Rat, Mouse, Chicken, Rabbit, Pig, Goat and Sheep.

Quick Overview for Smooth Muscle Actin antibody (ABIN959463)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Pig, Goat, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Clone: IA4

Product Details anti-Smooth Muscle Actin Antibody

Specificity: Reacts exclusively with alpha-smooth muscle actin, which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope is Ac-EEED, and is highly conserved. Therefore, the antibody cross-reacts with many species including protochordates, lower craniates and mammals.

Purification: Purified

Immunogen: Peptide comprising the first 10 amino acids of alpha-smooth muscle actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin via the C-terminal cysteine (Ac-EEEDSTALVC).

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, EM, IHC, IHC-Fr (1:10 - 1:100), WB (1:10000 - 1:50000)

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2 / Smooth Muscle Actin

Background: Name/Gene ID: ACTA2

Synonyms: ACTA2, ACTVS, Alpha-actin-2, Alpha-cardiac actin, AAT6, Actin, aortic smooth muscle, ACTSA, Smooth Muscle Actin, MYMY5

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development