Vimentin antibody

Catalog No. ABIN959513

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB, IHC, FACS, IHC (p), ICC and IHC (fro). It is suitable to detect Vimentin in samples from Human, Rat, Pig, Chicken and Sheep.

Quick Overview for Vimentin antibody (ABIN959513)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Pig, Chicken, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: V9

Product Details anti-Vimentin Antibody

Brand: IHC-plus™

Specificity: Reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Purification: Purified

Immunogen: Vimentin isolated from porcine lens

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:25 - 1:200), ICC, IHC, IHC-Fr (1:25 - 1:200), IHC-P (20 μg/mL), WB (1:100 - 1:1000)

Usage: For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (  pH 6.0) is required.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis