CA2 antibody

Catalog No. ABIN96020

Supplier Product No.: 100-4157

The Rabbit Polyclonal anti-CA2 antibody has been validated for WB and ELISA. It is suitable to detect CA2 in samples from Cow. There is 1 publication available.

Quick Overview for CA2 antibody (ABIN96020)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CA2 Antibody

Supplier Product No.: 100-4157

Supplier: Rockland

Purpose: Carbonic Anhydrase II Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Characteristics: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: This product has been assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CA2 antibody (ABIN96020)

Loussert-Fonta, Toullec, Paraecattil, Jeangros, Krueger, Escrig, Meibom: "Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section." in: Communications biology, Vol. 3, Issue 1, pp. 362, (2020) (PubMed).

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2

Background: Background: Carbonic Anhydrase 2 is essential for bone resorption and osteoclast differentiation. It reverses hydration of carbon dioxide and can hydrate cyanamide to urea. It is involved in the regulation of fluid secretion into the anterior chamber of the eye. Carbonic Anhydrase II contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It stimulates the chloride-bicarbonate exchange activity of SLC26A6. it is used for target of drugs used in treatments against glaucoma disorder and breast cancer.

Gene ID: 280740

NCBI Accession: NP_848667

UniProt: P00921