CA2 antibody

Catalog No. ABIN96021

Product Details anti-CA2 Antibody

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.

Purity: Anti-CARBONIC ANHYDRASE II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti CARBONIC ANHYDRASE II Antibody has been assayed against 1.0 µg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) [code #611-1302] and TMBE [code # TMBE-1000] as a substrate for 30 minutes at room temperature.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase II (CA2 Products)

Synonyms: CG6906 antibody, CT21171 antibody, Dmel\\CG6906 antibody, CA-2 antibody, caII antibody, car2 antibody, ca ii antibody, ca-ii antibody, BETA CA2 antibody, BETA CARBONIC ANHYDRASE 2 antibody, CA18 antibody, CARBONIC ANHYDRASE 18 antibody, CARBONIC ANHYDRASE 2 antibody, T9L3.40 antibody, T9L3_40 antibody, carbonic anhydrase 2 antibody, LOC100135827 antibody, AI131712 antibody, CAII antibody, Ca2 antibody, Car-2 antibody, Ltw-5 antibody, Lvtw-5 antibody, CA-II antibody, CAC antibody, Car2 antibody, CAR3 antibody, Carbonic anhydrase 2 antibody, carbonic anhydrase 2 L homeolog antibody, carbonic anhydrase 2 antibody, carbonic anhydrase 1 antibody, carbonic anhydrase II antibody, carbonic anhydrase, alpha type, periplasmic antibody, CAH2 antibody, ca2.L antibody, ca2 antibody, CA2 antibody, HCAG_04637 antibody, LOC100135827 antibody, LOC100635510 antibody, Car2 antibody, Ca2 antibody, LOC100154873 antibody, LOC101113341 antibody

Background: CARBONIC ANHYDRASE II Antibody is specific for carbonic anhydrase II. Carbonic anhydrases (or carbonate dehydratases) are a family of metalloenzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and Hydrogen (or vice versa). This reversible reaction occurs slowly in the absence of the carbonic anhydrase catalyst. The active site of most carbonic anhydrases contains a zinc ion cofactor. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Gene ID: 280740

UniProt: P00921