Cytochrome C antibody (AA 1-80) (Biotin)

Catalog No. ABIN960433

This Mouse Monoclonal antibody specifically detects Cytochrome C in WB, IHC and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Horse, Pigeon, Drosophila melanogaster and Pig.

Quick Overview for Cytochrome C antibody (AA 1-80) (Biotin) (ABIN960433)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse, Horse, Pigeon, Drosophila melanogaster, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 7H8-2C12

Product Details anti-Cytochrome C Antibody (Biotin)

Binding Specificity: AA 1-80

Specificity: An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached).

Purification: Protein G purified

Immunogen: Synthetic peptides corresponding to amino acids1-80,amino acids81-104,amino acids66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome c. based on competitive ELISA results (Jemmerson et al. 1991).

Type of Immunogen: Synthetic peptide

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB (0.05 - 0.5 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pigeon

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS / Cytochrome c

Background: Name/Gene ID: CYCS

Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity