alpha Tubulin antibody (C-Term, Subunit alpha)

Catalog No. ABIN962076

This anti-alpha Tubulin antibody is a Rat Monoclonal antibody detecting alpha Tubulin in WB, ELISA, IHC, IHC (p), IP, IHC (fro) and RIA. Suitable for Human, Saccharomyces cerevisiae, Mammalian, Avian and Echinodermata.

Quick Overview for alpha Tubulin antibody (C-Term, Subunit alpha) (ABIN962076)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Saccharomyces cerevisiae, Mammalian, Avian, Echinodermata

Host: Rat

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Clone: YL1-2

Product Details anti-alpha Tubulin Antibody

Binding Specificity: C-Term, Subunit alpha

Specificity: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

Purification: Affinity purified

Immunogen: Yeast tubulin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Pathways: Microtubule Dynamics