TRAF6 antibody (C-Term)

Catalog No. ABIN962140

This Rabbit Polyclonal antibody specifically detects TRAF6 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for TRAF6 antibody (C-Term) (ABIN962140)

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRAF6 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes human TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human and mouse TRAF6 (TNF receptor-associated factor 6) protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:200 - 1:400), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Immunoprecipitation: 7 μg/reaction. Positive control: Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates. Please note that cross-reactivity of this antibody with mouse has been confirmed only in WB. The antibody has not been evaluated for use in IHC with mouse samples at this time and suitability cannot be guaranteed.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Alternative Name: TRAF6

Background: Name/Gene ID: TRAF6

Synonyms: TRAF6, RING finger protein 85, MGC:3310, RNF85

Gene ID: 7189

UniProt: Q9Y4K3

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway