Vitamin D Receptor antibody (AA 89-105)

Catalog No. ABIN962160

The Rat Monoclonal anti-Vitamin D Receptor antibody (ABIN962160) specifically detects Vitamin D Receptor in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Vitamin D Receptor antibody (AA 89-105) (ABIN962160)

Target: Vitamin D Receptor (VDR)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Vitamin D Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vitamin D Receptor Antibody

Binding Specificity: AA 89-105

Specificity: Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.

Purification: Protein G purified

Immunogen: Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: LNCaP and HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: Vitamin D Receptor / VDR

Target Type: Chemical

Background: Name/Gene ID: VDR
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: VDR, NR1I1, Vitamin D3 receptor, Vitamin D receptor

Gene ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway