ATG4D antibody (AA 444-457)

Catalog No. ABIN962638

This Goat Polyclonal antibody specifically detects ATG4D in ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ATG4D antibody (AA 444-457) (ABIN962638)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4D Antibody

Binding Specificity: AA 444-457

Specificity: Human ATG4D.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Marmoset, Panda, Dog, Horse (93%) Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CSQLAQPTLRLPRT from an internal region (near C-terminus) of human ATG4D (NP_116274.3). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Marmoset, Panda, Dog, Horse (93%), Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx. 26  kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9  kDa according to NP_116274.3. The 26  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D

Background: Name/Gene ID: ATG4D

Synonyms: ATG4D, APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, Cysteine protease ATG4D, Autophagin-4

Gene ID: 84971

NCBI Accession: NP_116274

UniProt: Q86TL0

Pathways: Autophagy