ODF2 antibody

Catalog No. ABIN964607

Supplier Product No.: 200-401-c43

The Rabbit Polyclonal anti-ODF2 antibody has been validated for WB and ELISA. It is suitable to detect ODF2 in samples from Mouse.

Quick Overview for ODF2 antibody (ABIN964607)

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ODF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ODF2 Antibody

Supplier Product No.: 200-401-c43

Supplier: Rockland

Purpose: Cenexin-1 Antibody

Cross-Reactivity (Details): It is directed against, and shows specific reactivity for, human Cenexin-1 protein.

Characteristics: Synonyms: rabbit anti-Cenexin-1 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2 antibody, Cenexin1 variant 1 antibody, KKT 4 antibody, Cenexin 1

Purification: This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Cenexin-1 purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:500 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.53 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Alternative Name: ODF2

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1 (ODF84) and cenexin-2 are splicing variants that are expressed from the same genetic locus Outer Dense Fiber protein 2 (ODF2) . Cenexin-1 and cenexin-2, regarded as centriolar proteins. Cenexin-1, a 93-kD variant of ODF2, is the major form of cennexin expressed in various somatic cells and tissues and associates with centrosomes. Cenexin-1 contains a unique C-terminal extension that appears to play a critical role in recruiting mitotic regulators such as polo-like kinase 1. In contrast, ODF2, a 70- kDa protein, is a major component of sperm tail cytoskeleton and abundantly expressed in testis. Additional evidence suggests that both of these proteins play important role in primary cilia formation. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation.  Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene, however, not all isoforms and variants have been fully described.

Gene ID: 4957

NCBI Accession: NP_702915

UniProt: Q5BJF6

Pathways: M Phase